Effect of RBV on viral replication in seven cell lines.

<p>(A) The organization of the negative-sense RNA genomes of the recombinant viruses used in this study. (B) The panels show photographs of cells pretreated for 24 h with increasing concentrations of RBV as indicated (or mock-treated), infected with VSV-GFP (left) or SeV-GFP (right) at MOI 3 CIU/cell (or mock-infected, upper row), and then the same concentrations of drugs as in the pretreatment was added to each well after virus absorption. Fluorescence (upper panels) and light (lower panels) microscopy images were captured at 10× magnification. The photographs are typical representations of at least three independent experiments and an average field for each well is shown. (C) Media from the experiments described in B was collected at 24 h p.i for VSV (left) or at 48 h p.i for SeV (right) and virus titer was determined by standard plaque assay on BHK21 (for VSV) or Vero cells (for SeV). The data represent the mean ± standard deviation of two independent experiments (done in duplicates). Statistical analysis was done using one-way ANOVA with Tukey's post hoc test (GraphPad Prism 4, San Diego, CA). RBV treatments without significant decrease in viral titer at any tested RBV concentrations as compared to mock-treated cells (“0 µM RBV”) are indicated as <i>P</i>>0.05.