Plaque reduction assay to determine RBV inhibitory concentrations.

<p>Cell monolayers were infected with VSV-GFP or SeV-GFP (or mock-infected; 0 µM RBV) using virus dilutions producing about 100 virus (“100%”) on each cell line in the absence of RBV, overlaid with SFM containing 1.2% Avicel RC-581 and increasing concentrations of RBV (note that different RBV concentrations were used for each virus-cell type combination). Cells were then incubated for 24 h (VSV) or 48 h (SeV), and plaques were counted with the aid of fluorescence and bright field microscopy. “0%” indicates that no fluorescent infectious foci were detected. Each experiment was performed at least twice (done in duplicates) and data points represent the mean ± standard deviation.