FM-MSCs are fully permissive to VZV infection.

<p>(A) HEL or FM-MSCs were infected with VZV at a moi<1 pfu/cell, as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071412#s2" target="_blank">Materials and Methods</a> section. At the indicated time pi cells were analyzed by optical microscopy; (B) To determine if the cytopathic effect observed in (A) was due to productive VZV infection, infected cells were harvested at different time points pi along with their SNs, and processed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0071412#s2" target="_blank">Materials and Methods</a> to reinfect naive HEL cells. Infectious virus production was subsequently quantified by means of plaque counting. Each reported pfu value is referred to 10⁵ infected cells.