High glucose did not increase expression of TRPV1 channels.

<p>A, representative images immunocytochemistry for the detection of TRPV1 protein in cultured DRG maintained in either control (CTL) or high glucose (HG) conditions. B, bar graph summarizes mean pixel intensity for CTL (n = 71; HG, n = 51). C, western blots showing TRPV1 and β-actin expression in 4 independent samples (per treatment) of cultured DRG neurons exposed to either CTL or HG for 10 days. B, bar graph summarizes the ratio of TRPV1/β-actin per treatment. All data are represented as mean ± SEM. Scale bar in A represents 30 μm. Statistical analysis by Mann<i>–</i>Whitney test; no significant differences were found between treatments (B-D). AU, arbitrary units.