DRG neurons produce eotaxin-1, ICAM-1, and VCAM-1.

<p>(A) DRG neurons were isolated and cultured alone for 24 hours, then fixed and stained with anti-eotaxin-1 (red). Cell nuclei were counterstained using DAPI (blue). Two representative photomicrographs are shown. Scale bars = 50 um. (B–C) DRG neurons were cultured alone or with 40 ng/ml NGF for 24 hours, then fixed and stained with anti-ICAM-1 (B) or anti-VCAM-1 (C). Left, no primary with secondary negative control; center, DRG stained with antibody in cultures without exogenous NGF; right, DRG stained with antibody in cultures with NGF added. GRAPH: Quantification of relative fluorescence units, compared to background and to negative controls, was performed using Metamorph.