Dynasore and EIPA inhibit rVSV CVS G infection in compartmentalized neuronal culture.

<p>Fluorescence microscopy of MF cultures of <b>A.</b> DRG neurons or <b>B.</b> V SC neurons infected with rVSV CVS G in the N compartment. Neurons were additionally treated with the dynamin inhibitor, dynasore (150 μM), or the macropinocytosis inhibitor, EIPA (25 μM), as indicated at either 0 or 2 hpi and monitored for viral eGFP (green) expression at 26 hpi. Cells are further stained against phosphorylated neurofilament H (NF; red) and with DAPI (blue). <b>C.</b> Neurites in the N compartment at 26hpi and continuous treatment with the indicated inhibitor, stained against neurofilament H. <b>D.</b> Quantitation of percentage eGFP positive neurons in three iterations of the experiment in DRG culture. Error bars indicate the standard deviation for each condition.