Loss of SVOP does not affect spontaneous neurotransmitter release.

<p>Shown are graphs summarizing peak amplitude and frequency of mEPSC. Autaptic hippocampal neurons cultured from mice homozygous for Floxed SVOP genes or wild type littermate controls were infected with lenti virions encoding Cre-eGFP or eGFP. Neurons were analyzed in the whole-cell voltage-clamp configuration at DIV 14-20. Cells were selected for recording according to green fluorescence expression with preference given to brighter cells. Neurons were held at −60 mV, and mini EPSC were recorded in 5 min epochs. Graphs show the mean ± SEM. The number of cells analyzed is indicated within each column. Data are from three different cultures. <b>A)</b> Representative traces of mini EPSCs recorded from autaptic hippocampal neurons. <b>B)</b> Mean amplitudes of mEPSCs were unchanged across four experimental groups (SVOP^WT/WT - Cre, 24.78 ± 1.56 pA; SVOP^WT/WT + Cre, 24.74 ± 1.73 pA, SVOP^Flox/Flox -Cre 26.13 ± 1.10 pA and SVOP^Flox/Flox + Cre 26.28 ± 2.03 pA, <i>p</i> = 0.88, two-way ANOVA). <b>C)</b> Cumulative probability plots of mEPSC amplitude. The left panel shows the full amplitude range, the right panel is a plot of events with amplitudes < 80 pA. <b>D)</b> No differences in mEPSC frequency were observed across all experimental groups (SVOP^WT/WT - Cre, 3.76 ± 0.83 Hz; SVOP^WT/WT + Cre, 4.00 ± 0.77 Hz, SVOP^Flox/Flox -Cre 4.95 ± 1.06 Hz and SVOP^Flox/Flox + Cre 5.24 ± 1.58 Hz, <i>p</i> = 0.76, two-way ANOVA). <b>E)</b> Cumulative probability plots of mEPSC inter-event interval of 4 experimental groups. The left panel is a plot of the full inter-event interval range, the right panel of inter-event intervals <4000ms.