The loss of MEF2A/D does not alter basal synaptic transmission.

<p>(<b>A</b>) mEPSC frequency is unchanged upon deletion of <i>Mef2a/d</i> in hippocampal culture neurons using lentivirus expressing Cre recombinase (p>0.05). The number of recordings is shown in the bar graph for the GFP and Cre infected neurons. Data is shown as mean ± SEM. (<b>B</b>) Lentivirus containing either GFP or Cre was infected at 4 DIV in hippocampal culture neurons and cells were harvested at 15–18 DIV, in parallel with the time of recordings. Quantitative PCR was used to confirm the deletion of <i>Mef2a/d</i> (*p<0.05). Relative mRNA expression of <i>Mef2a/d</i> was normalized to GAPDH (GFP, n=6; Cre, n=6). Data is shown as mean ± SEM.