Cre recombinase disrupts SVOP expression in neurons from mice homozygous for the floxed SVOP gene.

<p><b>A</b>) Shown are representative Western blots of protein expression in primary hippocampal neurons cultured from newborn pups homozygous for the targeted SVOP gene, or from wild type pups. Neurons were infected with lenti virions encoding Cre-eGFP or eGFP. The expression level of SVOP and the indicated proteins were detected by Western blot analysis. β3-tubulin, a neuron-specific isoform of tubulin, was probed for as a loading control. <b>B</b>) Plots of Western blot results from two independent experiments. Each dot represents one datum, and a horizontal line indicates the mean. The signals for SVOP, SV2 and synaptotagmin were normalized to β3-tubulin in the same lane and expressed as a percentage of the wild type - Cre lane in the same blot. SVOP was dramatically decreased in SVOP^Flox/Flox neurons expressing Cre. Expression of the synaptic vesicle proteins synaptotagmin and SV2 was similar across conditions.