High glucose induces intracellular ROS accumulation.

<p>A, representative images of ROS detection by CM-H2DCFDA fluorescence, in cultured DRG neurons from WT and RAGE KO mice, maintained in control (CTL) and high glucose (HG) conditions for 1 week. B, the bar graph summarizes mean ± SEM pixel intensity for HG/CTL ratio for neurons from WT (n = 110) and RAGE KO (n = 156) mice, respectively. C, representative traces showing CAP-evoked currents recorded in the presence of ALA+CAT in CTL and HG conditions. D-F, bar graphs summarize maximal current (I_max) density (D), maximal current charge (Q_max; E), and current rundown calculated as the ratio I₁₅/ I_max (F). All data are represented as mean ± SEM, ALA + CAT (CTL, n = 10; HG, n = 12). Scale bar in A represent 30 μm. Statistical analysis by one sample <i>t</i>-test hypothetical mean = 1 (B), unpaired <i>t</i>-test (D-E), and Mann-Whitney test (F). ***, p < 0.001.