E-cadherin staining in 21-day skin equivalents.

<p>E-cadherin staining (green) in Ker-CT keratinocytes demonstrated the cellular connections reminiscent of stratum spinosum in human skin. Little staining was present on the basal surface of the basal layer. E-cadherin staining in H-Ras keratinocytes was less organized than Ker-CT although most of the interconnected cells were in the upper regions of the tissue. Ker-CT-Ras-T had little or no specific E-cadherin staining. Lower images are insets of corresponding upper images. Scale bar: 40 µm.