Immunohistochemical staining.

<p>(A) Immunohistological staining against keratin 10 (red) and keratin 5 (green) in neoepidermis with incorporated microcarriers and compared to (B) normal control skin. (C) Immunohistological staining against pancytokeratin (red) showing microcarrier populated by keratinocytes. (D) Immunohistological staining against laminin 5 (green) in neoepidermis with microcarriers, (E) unwounded skin, and (F) neoepidermis of control wound. Nuclear staining with 4, 6-diamidino-2-phenylindole (DAPI). All scale bars = 100 μm.