<p>Staining Pattern of Immunohistochemical Markers from representative areas of Non-Stored Cultured Limbal Epithelial Cell Sheets. The pictures show nuclear markers A) ΔNP63α, B) pan-p63, C) Bmi-1, and D) C/EBP∂; membrane markers E) ABCG2, and F) Cx-43; keratin markers G) K19, and H) K3, proliferating cell marker I) PCNA, and finally, apoptosis marker J) Caspase-3. Original magnification 400x. Arrow in C) marks positively stained cell.</p
<p>Human limbal epithelial cells were expanded on cover slides in plastic dishes with or without var...
Rhodamine staining of cultured limbal epithelial cells from large (A and C) and small (B and D) expl...
<p>Cells of the explant expansion showing BrdU (Red) staining denoting cell proliferation compared t...
<p>Bar chart of mean percentage of positively stained cells for 4 different storage conditions compa...
<p>Bar chart of mean percentage of positively stained cells for 4 different storage conditions compa...
Immunostaining (A and B) and Quantification (C) of proliferation markers. A, and B: Immunostaining o...
<p>Bar chart of mean percentage of positively stained cells for 4 different storage conditions compa...
Immunostaining (A and B) and Quantification (C) of immature phenotype markers. A and B. Immunostaini...
<p>Limbal explant and proximal outgrowing cells stained for p63α and/or Vim (A, B, C), and distal gr...
<p>A) BAD (brown) is expressed in the epithelium (E), most prominently towards the lumen (L), of the...
<p>Limbal explant and outgrowing cells proximal (A, C, E), and 3D cell outgrowth distal to the expla...
<p>(A) Epitheloid cell sheets under airlifting culture. (B) HE staining of epitheloid cell sheet. (C...
<p>a) Immunofluorescence staining of cytokeratin 5 (blue is DNA; green is CK5; white is transmitted ...
<p>Limbal explants and outgrowing cells proximal (A, C) and distal from it (B, D) being stained for ...
PURPOSE. To characterize human limbal epithelial cells based on the expression levels of nuclear pro...
<p>Human limbal epithelial cells were expanded on cover slides in plastic dishes with or without var...
Rhodamine staining of cultured limbal epithelial cells from large (A and C) and small (B and D) expl...
<p>Cells of the explant expansion showing BrdU (Red) staining denoting cell proliferation compared t...
<p>Bar chart of mean percentage of positively stained cells for 4 different storage conditions compa...
<p>Bar chart of mean percentage of positively stained cells for 4 different storage conditions compa...
Immunostaining (A and B) and Quantification (C) of proliferation markers. A, and B: Immunostaining o...
<p>Bar chart of mean percentage of positively stained cells for 4 different storage conditions compa...
Immunostaining (A and B) and Quantification (C) of immature phenotype markers. A and B. Immunostaini...
<p>Limbal explant and proximal outgrowing cells stained for p63α and/or Vim (A, B, C), and distal gr...
<p>A) BAD (brown) is expressed in the epithelium (E), most prominently towards the lumen (L), of the...
<p>Limbal explant and outgrowing cells proximal (A, C, E), and 3D cell outgrowth distal to the expla...
<p>(A) Epitheloid cell sheets under airlifting culture. (B) HE staining of epitheloid cell sheet. (C...
<p>a) Immunofluorescence staining of cytokeratin 5 (blue is DNA; green is CK5; white is transmitted ...
<p>Limbal explants and outgrowing cells proximal (A, C) and distal from it (B, D) being stained for ...
PURPOSE. To characterize human limbal epithelial cells based on the expression levels of nuclear pro...
<p>Human limbal epithelial cells were expanded on cover slides in plastic dishes with or without var...
Rhodamine staining of cultured limbal epithelial cells from large (A and C) and small (B and D) expl...
<p>Cells of the explant expansion showing BrdU (Red) staining denoting cell proliferation compared t...