Effects of explant size on epithelial outgrowth, thickness, stratification, ultrastructure and phenotype of cultured limbal epithelial cells - Fig 9

Immunostaining (A and B) and Quantification (C) of proliferation markers. A, and B: Immunostaining of 1) Ki67, and 2) PCNA of cultured human LEC derived from small (A) and large (B) limbal explants. The selected areas in the micrographs are representative for the staining and not for the epithelial thickness in the study groups. Scale bar = 100 μm. C: Bar charts showing the percentage of positive cells for the proliferation markers in cultured LEC sheets cells derived from small (grey) and large (black) explants. Error bars represent the standard deviation of mean values.