Double-staining of vimentin and p63 in 21-day skin equivalents.

<p>p63 (red or pink) and vimentin (green) rarely co-localized in Ker-CT keratinocytes, except in occasional basal cells (arrow). Fibroblasts (arrowhead) were identified by the blue nucleus and green cytoplasm. More co-localization was seen in H-Ras keratinocytes, although primarily in the lower strata. Note the lack of either staining in superficial strata. Lower images are insets of corresponding upper images. Scale bar: 40 µm.