Reduction of WGEF mRNA expression during adipogenesis.

<p>A. Representative cell morphology of 3T3-L1 cells at days 1, 3, 5 and 9 of differentiation culture. Lipid droplets appeared by days 5 of culture. B. Quantitative real-time RT-PCR was performed for WGEF, PPAR-γ, Adipsin and Gapdh. Relative expression of each gene was estimated at days 1, 3, 5, 7 and 9 of differentiation culture. The β-actin gene was used to standardize the data. Data represent the mean +/− standard deviations in triplicate. C. WGEF mRNA distribution in mouse tissues. Quantitative real-time RT-PCR was performed for the brain (B), heart (H), kidney (K), lung (Lu), skeletal muscle (SM), intestine (I), spleen (Sp), thymus (Th), stomach (St), fat (F) and testis (Te). The β-actin gene was used to standardize the data. Data are the mean +/− standard deviations in triplicate. D. WGEF mRNA expression in white adipose tissue of control mice or high-fat diet (HFD)-induced obese mice (n = 6, respectively). The β-actin gene was used to standardize the data. Data are the mean +/− standard deviations in triplicate: *<i>P</i><0.05