WGEF inhibits adipogenesis in 3T3-L1 cells.

<p>A. Expression vectors with/without WGEF cDNA were individually transfected into 3T3-L1 cells, which were subsequently selected in G418-containing medium. Then, GFP-positive clones were analyzed. B. Quantitative real-time RT-PCR was performed for total (endogenous and transgenic) WGEF. Relative expression of each cell line was estimated at day 9 of differentiation culture. The β-actin gene was used to standardize the data. Data are the mean +/− standard deviations in triplicate. C. Oil Red O staining of WGEF- and vector-transfected 3T3-L1 cells subjected to 9 days of differentiation. Vector control cells exhibited significantly more stained lipid droplets than WGEF-transfected cells, except for the W8 cell line which showed less than 50% WGEF expression compared to undifferentiated No TG cells. D. Cells with high expression of WGEF (GFP) were not differentiated, whereas those with a low expression of WGEF (GFP) differentiated and produced lipid granules (arrows) even in the same cell line.