Expression analysis in adipose tissues of genes involved in lipid metabolism.

<p>Relative mRNA levels of Glut4, FASn, ABHD5 and ATGL were determined by real-time PCR experiments, in subcutaneous (SCAT) and visceral adipose tissues (VAT) of control □ and SBA ▪ mice. PPIA and HPRT were used as housekeeping genes. All results are expressed as fold-change compared to one SCAT of the control group after 10 weeks. Analyses were done after 10 weeks of SBA protocol and 10 additional weeks of REC protocol. Data represent mean ± SEM; n = 5–10/group. *<i>p</i><0.05 and **<i>p</i><0.005 when compared to CT group at the same duration; ^‡<i>p</i><0.05 and ^{‡ ‡}<i>p</i><0.005 when compared to the previous value of the same group.