TD antigen-specific immunoglobulin and long-lived plasma cell production are impaired in TRAF6-ΔB mice.

<p>(a–c) Defective antigen-specific IgG1 and IgG2b production in response to TD antigens for primary response to the TD Ag NP-KLH (“1^st”, 7days after immunization) and also for the secondary response (“2^nd”, 14 days after immunization as detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004736#s4" target="_blank">Methods</a>). The level of high-affinity (clear bars) and total (black bars) Abs are compared: (a), antigen-specific IgM levels; (b), IgG1; (c), IgG2b. Inset in (b) shows the primary response plotted on a smaller scale. Data are presented as mean±SD. *; <i>p</i><0.05, compare total and high affinity Igs, **; <i>p</i><0.05, compare control and TRAF6-ΔB mice. (c) Reduced number of long-lived plasma cells producing NP-specific IgG1 in the BM of TRAF6-ΔB mice collected 60 days after immunization (as described in a–c), detected by ELISpot assay.