MyoR is dispensable for T-dependent humoral responses.

<p>(A–F) WT and MyoR^−/− mice were immunized against NP-KLH/Alum (A–D) or KLH/Alum (E, F) and tested for Tfh cell marker expression in the draining lymph nodes. Representative contour plots showed the expression of CXCR5⁺PD-1⁺ (A), CXCR5⁺ICOS⁺ (C) or CXCR5⁺ BCL-6 ⁺ (E) among CD4⁺ T cells. Histograms in (B, D, F) represent the mean ± SD of marker-positive cells from 4 individual mice and are representative of 3 independent experiments. (G) Aliquots of cells were stimulated for 4 h with PMA and ionomycin and tested for IL-21 production by intracellular staining. Histograms represent the mean ± SD of IL-21⁺ cells in the Tfh (CXCR5⁺PD-1⁺) and non-Tfh (CXCR5⁻PD-1⁻) subsets of WT and MyoR^−/− mice (4 mice/group). (H) NP-specific serum IgG1 titers of WT (open symbols) and MyoR^−/− (closed symbols) mice were determined at different timings after i.p. immunization with NP-KLH/Alum. (I) sera from panel H were tested for NP-affinity. Relative affinities are expressed as ratio of 50% binding on NP₂-BSA and NP₃₀-BSA- coated plates. Each dot represents a mouse. Data are representative of two independent experiments. *: p<0.05; **: p<0.01; ns, not significant; n.i., non immune mouse.