Knock-down of ABCG2 led to downregulation of Nanog.

<p>(A–D) Immunofluorescence staining for mES-R1 cells transfected with control scramble construct (A, B) and shABCG2-3 construct (C, D) and. The GFP marked transfected cells and red represented ABCG2 (A, C) and Nanog (B, D) staining. Immunofluorescence staining was performed at 72 hours after transfection. Images represent transfections with either shABCG2-2 or shABCG2-3. (E) RT-PCR data for Oct-4, Nanog and ABCG2 for mES-R1 cells transfected with scrambled, shABCG2-2 or shABCG2-3 with GAPDH as internal control. (F, G) Real-time PCR data showing relative mRNA expression level of ABCG2 (F) and Nanog (G) in mES-R1 cells transfected with scrambled, shABCG2-2 or shABCG2-3 normalized to GAPDH. A t-test was performed to confirm statistical significance and * indicates p<0.05 and ** indicates p<0.01. Data was calculated from at least three independent experiments.