Expression of ABC transporter genes.

(A) RT-PCR analysis of PANC-1 clone cells. The expression of ABC transporter genes, NANOG, CDH1 and CDH2 in PANC-1 W/T cells, clone #2 cells, clone #4 cells, and clone #6 cells was analyzed by RT-PCR. GAPDH was used as an internal positive control. (B) RT-PCR analysis of gastrointestinal cancer cells. PEPT1, PEPT2, ABCB6 and ABCG2 expression was analyzed by RT-PCR. GAPDH was used as an internal positive control. (C) qRT-PCR analysis of ABCG2 expression. ABCG2 expression was analyzed by qRT-PCR. Data are shown as the mean ± standard deviation. (D) Correlation of expressions of ABCG2 and %MFI increase. Correlation of expression of quantitative ABCG2 expressions of gastrointestinal cancer cell lines and %MFI increase was analyzed by Pearson’s correlation coefficient. (E) Correlation of expressions of ABCG2 and %MFI increase. Correlation of expression of quantitative ABCG2 expressions of PANC-1 clone cells and %MFI increase was analyzed by Pearson’s correlation coefficient.