Overexpression of ABCG2 did not alter the level of Nanog and Oct-4.

<p>(A, B) Immunofluorescence staining for mES-R1 cells transfected with CMS-ABCG2. The GFP marks transfected cells and red represents Oct-4 (A) and Nanog (B) Immunofluorescence staining at 72 hours after transfection. (C) RT-PCR data for Oct-4, Nanog and ABCG2 for mES-R1 cells transfected with control vector or CMS-ABCG2 with GAPDH as internal control. (D) Real-time PCR showing relative mRNA expression level of ABCG2 in mES-R1 cells transfected with control vector or CMS-ABCG2 normalized to GAPDH. Data was calculated from at least three independent experiments.