Stability of GC-Rich and GC-Poor mRNA in Mammalian Cells

<p>HeLa cells were transfected with the indicated plasmids and after 20 h they were treated with 10 μg/mL actinomycin D. At the indicated times, mRNA was isolated and quantified by real-time RT-PCR. The GAPDH and c-myc mRNA levels represent the means of their levels in cells transfected with GC-rich and GC-poor genes. In each graph, little circles represent GAPDH and crosses, c-myc. (A) black circles, Hsp70; white circles, Hsc70. (B) black squares, EGFP; white squares, GFP. (C) Black triangles, eIL2; white triangles, IL2. The data is representative of two independent experiments.