MHC-I mRNA expression in fibroblasts isolated from control and NOD mice.

<p>Cells were treated with 0 or 1000 U/ml of IFN-γ for 48 hours. <b>A</b>: RT-PCR analysis of MHC-I mRNA expression. <b>B</b>: the Mean±SEM ratio of densities of MHC-I to GAPDH. Solid and open bars represent C57BL/6 and NOD fibroblasts respectively. GAPDH was used as loading control. *denotes significant difference between C57BL/6 and NOD fibroblasts treated with IFN-γ in terms of MHC-I expression (n = 3, p<0.05). **corresponds to significant difference between cells from the same strain treated with 0 or 1000 U/ml of IFN-γ (n = 3, p<0.01).