<p>Transcript levels of six genes encoding putative gamete-enriched proteins and of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in somatic tissues and gametes were monitored by quantitative PCR and normalized to ubiquitin mRNA. The data are means ±SE of two independent experiments. The numbers at the bars indicate values of relative mRNA levels. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0069578#pone.0069578.s004" target="_blank">Table S1</a> for primer sequences.</p
The study of mammalian gene expres-sion is often carried out at the level of mRNA. In such analyses,...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used...
<p>Note: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GCN5, general control non-derepressible 5;...
<p>The expression values generated by RNA-seq (red) or qPCR (blu) for eleven selected genes using th...
<p>Real-time PCR showing relative expression levels of <i>SSTR2</i> (A) and <i>NCL</i> (B) in a pane...
<p>mRNA levels were quantified by qRT-PCR and corrected by the level of GAPDH on each sample as a ho...
<p>Expression of 14 genes was measured in individual gonad samples from Site 1 (Locmariaquer, Britta...
<p><sup>a</sup> The relative-fold expression determined by Illumina transcriptome analysis.</p><p><s...
<p>HeLa cells were transfected with the indicated plasmids and after 20 h they were treated with 10 ...
<p>For each sample, mRNA expression level was normalized to the level of GAPDH housekeeping genes us...
<p>Panels A–F show expression values from quantitative RT-PCR (qRT-PCR) experiments for indicated ge...
<p>11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; ...
<p>Collagen 1 α 1 (Col1a1), Fibronectin 1 (FN1), and thrombospondin 1 (THBS1) were highly upregulate...
The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique ...
<p>Determination of transcript levels by means of quantitative real-time RT-PCR. Investigation of GF...
The study of mammalian gene expres-sion is often carried out at the level of mRNA. In such analyses,...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used...
<p>Note: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GCN5, general control non-derepressible 5;...
<p>The expression values generated by RNA-seq (red) or qPCR (blu) for eleven selected genes using th...
<p>Real-time PCR showing relative expression levels of <i>SSTR2</i> (A) and <i>NCL</i> (B) in a pane...
<p>mRNA levels were quantified by qRT-PCR and corrected by the level of GAPDH on each sample as a ho...
<p>Expression of 14 genes was measured in individual gonad samples from Site 1 (Locmariaquer, Britta...
<p><sup>a</sup> The relative-fold expression determined by Illumina transcriptome analysis.</p><p><s...
<p>HeLa cells were transfected with the indicated plasmids and after 20 h they were treated with 10 ...
<p>For each sample, mRNA expression level was normalized to the level of GAPDH housekeeping genes us...
<p>Panels A–F show expression values from quantitative RT-PCR (qRT-PCR) experiments for indicated ge...
<p>11β-HSD1, 11β-hydroxysteroid dehydrogenase type 1; 5αR1, 5α-reductase type 1; 5βR, 5β-reductase; ...
<p>Collagen 1 α 1 (Col1a1), Fibronectin 1 (FN1), and thrombospondin 1 (THBS1) were highly upregulate...
The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique ...
<p>Determination of transcript levels by means of quantitative real-time RT-PCR. Investigation of GF...
The study of mammalian gene expres-sion is often carried out at the level of mRNA. In such analyses,...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) mRNA level quantitation is widely used...
<p>Note: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GCN5, general control non-derepressible 5;...