Expression of GFP and IL2 Variants in Stably Transfected MCF-7 Cells

<p>MCF-7 cells were stably transfected with expression plasmids containing GFP (GC3 = 35%), EGFP (GC3 = 96%), wIL2 (GC3 = 7%), IL2 (GC3 = 41%), IL2-eIL2 (GC3 = 70%), eIL2 (GC3 = 100%), or with an empty pcDNA3.1 plasmid. The expression plasmids contained CMV promoters and were integrated in random genomic locations. Protein and mRNA was quantified in three to five individual clones for each transgene. (A) Flow cytometry measurements of GFP and EGFP protein levels. (B) Real-time RT-PCR measurements of GFP and EGFP mRNA levels. (C) ELISA measurements of the IL2 protein levels. (D) real-time RT-PCR measurements of IL2 mRNA levels. GFP and IL2 mRNA levels were normalized to GAPDH mRNA. The controls represent pcDNA3.1-transfected cells. The vertical axis in each graph represents arbitrary units.