Wounding induces c-myc mRNA accumulation in IEC18 cell monolayers.

<p>Rat intestinal epithelial IEC18 cells were grown to confluency in 6-well-plates, serum-starved overnight, and cell monolayers were then wounded by multiple scraping. At the indicated time points, total cell RNA extracts were prepared and analysed for c-myc mRNA accumulation normalized to GAPDH control via real-time RT-PCR using specific primers. mRNA accumulation relative to unwounded control is expressed as median and interquartile range (IQR) of n = 3 separate experiments, each with three separate wells per condition. Statistical analysis was performed by the Kruskal-Wallis test for non-parametric data (*p<0.05 versus control).