<i>Mφ-c-Myc-KO</i> mice exhibit reduced c-MYC expression in macrophages.

<p>(<b>A</b>) <i>c-M</i>yc mRNA expression determined by qPCR in peritoneal macrophages from control and <i>Mφ-c-Myc-KO</i> mice (n = 7 per genotype). (<b>B</b>) Total numbers of peritoneal macrophages isolated from control and <i>Mφ-c-Myc-KO</i> mice after washing the peritoneal cavity with 3 ml PBS (n = 8 mice per genotype). (<b>C</b>) Expression levels of <i>c-Myc</i> mRNA (top, n = 6 per genotype) and c-MYC protein (bottom, representative blot) in BMDMs. (<b>D</b>) <i>n-M</i>yc mRNA expression determined by qPCR in BMDMs from control and <i>Mφ-c-Myc-KO</i> mice (n = 4 per genotype). (<b>E</b>) Expression levels of <i>c-Myc</i> mRNA (top) and protein analyzed by western blot (bottom) in liver, kidney and testis (n = 3 control, n = 6 <i>Mφ-c-Myc-KO</i>). (<b>F</b>) BMDM proliferation assessed by 5′-bromodeoxyuridine (BrdU) incorporation (n = 3 per genotype).