Generation of the EGFP-Fmn1-IV fusion allele.

<p>(A) Diagram of the strategy for generating an in-frame insertion of EGFP into the Fmn1-IV locus, which produces an EGFP-tagged Fmn1-IV driven from the endogenous promoter in mice. The star designates the start of translation. The black filled boxes flanking the PGK Neo cassette represent LoxP sites. (B) Representative PCR genotyping of tail DNA from mice possessing the EGFP-Fmn1-IV allele are shown. The letter F indicates the fusion allele, while + indicates wt allele.