PCR-based genotype analysis.

<p>The generation of transgenic mice containing the <i>FXN-EGFP</i> genomic reporter construct in a murine <i>Fxn</i> knockout background was analyzed by PCR. The 615 bp band corresponds to the EGFP sequence in the <i>FXN-EGFP</i> transgene; the 520 bp band corresponds to the endogenous wild type murine <i>Fxn</i> gene; the 245 bp band corresponds to the <i>Fxn</i> knockout allele. C57BL/6, wild-type mouse (<i>Fxn</i>^+/+); Heterozygous KO, (<i>Fxn</i>^+/−); Primary transgenic, (<i>FXN-EGFP</i>⁺, <i>Fxn</i>^+/+); Double heterozygous, (<i>FXN-EGFP</i>⁺, <i>Fxn</i>^+/−); Rescue, (<i>FXN-EGFP</i>⁺, <i>Fxn</i>^−/−).