Generation of FDD_KI mice.

<p>1a. Schematic representation of the construct that was injected in 129 ES cells, showing site of ADan mutation on Exon 6, primer sites, site of Southern Blot probe, LoxP, pgk-dt and pgk-Neo sites. The bottom graphics depict the construct with and without the pgk-Neo cassette that has been removed by means of Cre recombinase. 1b. Examples of 2 ES clones positive for the homologous recombination of the mutated allele: left arm (a–c: 1.67 kb) and right arm (b–d: 3.4 kb) PCRs are shown. 1c. Southern Blot showing a shift from the 11.9 kb of the wild type genome to the 8.9 kb band of the FDD KI mice, due to the insertion of a new <i>BamHI</i> site. KI mice show both bands, indicating heterozygosity.