Transient and stable transgene expression of the flavin mononucleotide-based fluorescent protein FbFP in tumor cells and mouse neural stem cells.

<p>In a: schematic drawing of the FbFP vector is shown. The table in the vector map summarises the amount of base pairs and the plasmid backbones (pcDNA3.1 and pEF6) of al vectors used in this study (PP1 and BS2). In b: transiently transfected tumor cells lines from different species are shown (HEK cells; human embryonic kidney cells, CHO; chinese hamster ovarial cells, N2A; mouse neuroblastom-2a). The fluorescence of two different FbFPs (PP1 and BS2) each with the CMV or the EF1α promoter is compared to the fluorescence of eGFP (with CMV promoter as control). In c: two stably transfected neural cell populations are shown. In the upper row, a tumor cell line (HT22) and, in the lower row, mouse ES-cell derived neural stem cells (msNSC) are given. The fluorescence of two different FbFPs (PP1 and BS2) each with the CMV or EF1α promoter is compared to the fluorescence of eGFP (with CMV promoter as control).