Characterization of double transgenic GFAP/EGFP/α-Syn^−/− mice.

<p>(<b>A</b>) PCR of tail genomic DNA isolated from α-Syn^−/− mice, GFAP/EGFP mice, or double transgenic GFAP/EGFP/α-Syn^−/− mice. Mice lacking the <i>Snta1</i> gene coding α-syntrophin also express <i>Neo</i>, the gene of neomycin resistance, inserted in the gene construct as a positive selectable marker during homologous recombination (top). Western blot analysis detecting α-syntrophin in cortical tissue isolated from α-Syn^−/− mice, GFAP/EGFP mice, or GFAP/EGFP/α-Syn^−/− mice (bottom). (<b>B</b>) Immunohistochemical staining for aquaporin 4 (AQP4) in cortical slices isolated from α-Syn^−/− mice, (<b>C</b>) GFAP/EGFP mice or GFAP/EGFP/α-Syn^−/− mice. Note that α-syntrophin deletion is accompanied by the loss of AQP4 expression on the astrocytic membranes contacting blood vessels.