2-APB decreased the expression of maturation markers in human DC.

<p>In panel <b>A</b>, DC were cultured in medium with maturating agents (TG (750 nM), LPS (50 ng/ml), Zymosan (25 µg/ml) or TNF-α (20 ng/ml)) in the absence or presence of 2-APB (mean of percentage ± SD, *p<0,05, n = 7). In panel <b>B</b>, DC were cultured in medium with the maturating agents in the absence or presence of SKF 96365 (100 µM) (n = 7 for DCi, TG and LPS conditions and n = 4 for Zymosan and TNF-α). The marker expressions were analyzed by FACS as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061595#pone-0061595-g001" target="_blank">figure 1B</a>. In panel C, ORAI-1 and STIM-1 protein expressions were analyzed by western blotting to control Si-RNA efficacy.