Orai-1 and STIM-1 inhibition decreased the maturation marker expressions on DC.

<p>DC treated by si-Ctl (white), by si-STIM-1 (light grey) or by si-Orai-1 (dark grey) were maturated by following maturating agents (TG at 750 nM, LPS at 50 ng/ml, zymosan at 25 µg/ml or TNF-α at 20 ng/ml) for 18 h. Then, cells were harvested and quadruple staining (CD80, CD86, CD83, CD25) was assessed by FACS analysis for each condition. The results represent the mean of percentage of quadruple positive cells from 5 independent experiments (mean ± SD, *p<0,05).