Effect of the specific inhibitors of kinase signaling on the phenotypic maturation of DCs induced by particle-bound PADRE.

<p>Day-5 DCs were incubated for 2 hours with inhibitors for p72 Syk, PI3K, p38 MAPK or NF-κB after which particle-bound PADRE, particle-bound P2C or soluble LPS were added and the cultures continued for 2 more days. On day 7 the cells were analyzed by flow cytometry for expression of CD80, CD86, CD83 and HLA-DR and their culture supernatant by ELISA for IL-12p70. The graphs show fold change of expression of CD80 (<b>A</b>), CD86 (<b>B</b>), CD83 (<b>C</b>) and HLA-DR (<b>D</b>), or IL-12p70 levels in the supernatants (<b>E</b>) with standard error of 7 independent experiments with cells from 7 different healthy donors. * = p<0.05; ** = p<0.01; and *** = p<0.001 comparing the group treated with stimulus only vs. the groups treated with stimulus plus the specific inhibitor.