Ag85B-BCG-CWS induces DC maturation.

(A) DCs were dose-dependently treated with the indicated concentrations of MPL or BCG-CWS for 24 h. TNF-α, IL-6, IL-1β, IL12p70, and IL-10 levels in the culture medium were measured by an ELISA. (B) DCs were treated with the indicated concentrations of Ag85B, MPL, MPL+Ag85B, BCG-CWS, or Ag85B-BCG-CWS for 24 h. TNF-α, IL-6, IL-1β, IL-12p70, IL-23, and IL-10 levels in the culture medium were measured by an ELISA. (C) DCs were stained with anti-CD80, anti-CD86, anti-MHC class I, or anti-MHC class II mAbs and analyzed for the expression of surface markers. The frequency of positive cells is shown for each panel. All bar graphs show the means ± SD of 3 samples. One representative plot out of three independent experiments is shown; **p p < 0.001 compared to control group.