Inhibition of proliferation by TMZ or CAP treatment.

<p><b>A</b> Immunoblotting of the investigated cell lines for expression of the MGMT protein. Lysates of U87MG, LN18 and LN229 cells were blotted against the anti-MGMT antibody, GAPDH served as a loading control. MGMT protein is expressed in the LN18 cell line, U87MG and LN229 cells do not express the MGMT protein. <b>B</b> U87MG, LN229 and LN18 glioma cells were treated with 50 µM, 100 µM and 200 µM TMZ for three days consecutively. The number of viable cells was measured using the MTT assay after the treatment with TMZ. Statistical significances are evaluated for 200 µM of TMZ in the LN229 and U87MG cell lines. P-value: 0.001 <b>C</b> The same cell lines were treated with CAP in a 96-well plate without medium. Fresh medium was added to the cells immediately after the CAP treatment. Cell growth was measured 48 h after CAP treatment. Statistical significances were observed after 60 seconds of treatment in all tested cell lines. P-value *** <0.001.