Analysis of MGMT promoter methylation and MGMT expression levels.

<p>A) Modulation of the MGMT promoter methylation percentage in tumor cell lines before and after TMZ treatment. B) Real-time PCR analysis of MGMT expression before and after TMZ treatment. Showing relative expression of MGMT compared with SK-N-H cell line. C) Western blot analysis of MGMT protein expression in tumor cell lines before and after TMZ treatment. Beta-actin expression was used as a control. The graphs on the right show the densitometry of the MGMT Western blot analysis in SF268 and SK-N-SH cell lines before and after TMZ treatment. B: basal cells; 1C: first TMZ cycle; 2C: second TMZ cycle. All data represent the mean value ± SD of triplicate cultures.