Effect of TMZ on cell survival.

<p>Representative images of (A) U87-MG and U–SC, and (B) L0627 morphology after 48 h treatment with vehicle (0.1% DMSO) or 100 µM TMZ (+ TMZ). Images were viewed on a phase contrast microscope, and digital images were acquired (magnification, 10X; scale bar, 100 µm). (C) U87-MG and U–SC, and (D) L0627 cells were exposed to different concentrations of TMZ or vehicle. Cell viability was assessed after 48 h of treatment by MTT assay. Results are expressed as percentage of cell viability with respect to vehicle-treated cells (100%). Data are the mean ± SD of three independent experiments. * p < 0.05; ** p < 0.01 vs vehicle-treated cells. (E) Cell lysates (60 µg of proteins) were analyzed by immunoblotting with anti-MGMT and anti-β-actin antibodies. 98G human glioblastoma cell lysates were used as control. The immunoblottings are representative of one out of three.