Effect of combined repeated treatment with PROG and TMZ on the viability of U87MG and U118MG cell lines.

<p>Cells were grown in 24-well plate and repeatedly treated with PROG and TMZ at different concentration for 3 and 6 days. For repeated exposure, culture medium was replaced daily and the drugs were added to the medium every day. On day 4 and 7, cell viability test was performed using MTT reduction assay. PROG and TMZ stocks were prepared in absolute DMSO and further diluted in culture medium. The final concentration of DMSO was kept at <5μl/ml. Data are expressed as means ± SD of three separate replication experiments (n = 3 each). Statistically significant difference: *<i>P</i><0.05 compared with control group; ^#<i>P</i><0.05 compared to T100 alone group. P5 = PROG (5 μM); P80 = PROG (80 μM); T100 = TMZ (100 μM).