Other proteasomal inhibitors decrease TC-83 multiplication in U87MG cells.

<p>A) U87MG cells were seeded in a 96-well plate at a density of 10,000 cells per well. Cells were pre-treated with MG132 at 0.01μM, 0.1μM, or 1μM concentrations for 2 hours. Treated cells were infected with TC-83 at MOI: 0.1 for 1 hour. Supernatants were collected 24 hours post infection and infectious viral titers (PFU/mL) were assessed by plaque assay. B) U87MG cells were seeded in a 96-well plate at a density of 10,000 cells per well. Cells were treated with either DMSO or varying concentrations of MG132. Cell viability was determined 24 hours post-treatment using Cell-Titer-Glo Reagent as per manufacturer’s instructions. The graphs are representative of 3 independent experiments, each performed in triplicate. Standard deviations were calculated from 3 independent experiments and are represented thusly. C) U87MG cells were seeded in a 96-well plate at a density of 10,000 cells per well. Cells were infected in triplicate with TC-83 at MOI: 0.1 for 1 hour. Infected cells were treated with 1μM of MG132 at 2 hour intervals from 0–8 hours post infection. As controls infected cells were treated with DMSO. Supernatants were collected 24 hours post infection and analyzed by plaque assay for infectious viral particles (PFU/mL). The arithmetic means are illustrated graphically. The graph is representative of 2 independent experiments performed in triplicate. Standard deviations were calculated accordingly. p<0.05(*).