Time courses of the expression of FcγRs of PMNs after pre-inoculation with PRRSV strains.

<p>PMNs were isolated serially and FcγRs mRNA was measured by real-time PCR, and normalized relative to the expression of GAPDH. Results are shown as mean ± SE (n = 5). A. The expression of FcγRIIB. B. The expression of FcγRIIIA. The formula for calculating FcγRs modulation was Δ2^-ΔΔCT: Δ2^-ΔΔCT = 2^-ΔΔCT_inoculation-2^-ΔΔCT_{mock-infected control}.2^{−ΔΔCT =}2^{-(CT, FcγR-CT, GAPDH) Time x-(CT, FcγR-CT, GAPDH) Time 0}. *, p<0.05. **, p<0.01.