<p>Transcript alteration of three selected genes in PAM cells from the PRRSV-infected group compared with the mock-infected group. Total RNA extracted from PAM cells was measured by real-time RT-PCR analysis; relative expression levels were calculated according to the 2<sup>ΔΔ</sup>CT method, using β-actin as an internal reference gene and the mock-infected group as a calibrator (relative expression = 1). Error bars represent the standard deviation. Please refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085767#pone-0085767-t002" target="_blank">Table 2</a> for the identification of gene symbols that represent different genes.</p
<p>(A) (i) m-RNA expression (ii) quantification of Caspase 8, Bid, and Bax gene by RT-PCR in K562 ce...
<p>The relative expression levels were calculated using β-actin and GAPDH as the internal controls. ...
<p>The RT-PCR results from RNA isolated from PBMCs infected with HIV-1(MN) and HIV-2 (ROD) <i>(</i>A...
We conducted research on high-flux transcriptome regarding alternative polyadenylation and host gene...
<p>Twelve transcripts were selected for real-time RT-PCR to validate the expression patterns in diff...
<p>The heatmaps of 6,624 significant DEGs (left) and numbers of potential signature genes were group...
<p>(A) Real-Time RT-PCR analysis performed with RNA extracted from the PT4 cell culture containing G...
Murine L929 cells were infected for 19.5 h with reovirus isolates c87, c8 and Dearing or mock (M) in...
<p>(A) Scatter plot of global gene expression in the EtOH- and R5020- treated T47D cells. In total, ...
<p>All genes (A), up-regulated genes (B) and down-regulated genes at four time-points post-infection...
<p>Relative transcription of Th1, Th2, pro-inflammatory, anti-inflammatory, Th17 and regulatory cyto...
<p>Ten expression trend clusters of 699 DE genes derived from PAMs during PRRSV infection.</p
<p>Twelve expression levels of selected genes from the microarray assay were validated by real-time ...
<p>a. The ΔC<sub>T</sub> value is determined by subtracting the average Actin C<sub>T</sub> value fr...
1<p>fold change by real-time RT-PCR is relative gene expression level (chGH/PBS).</p>2<p>fold change...
<p>(A) (i) m-RNA expression (ii) quantification of Caspase 8, Bid, and Bax gene by RT-PCR in K562 ce...
<p>The relative expression levels were calculated using β-actin and GAPDH as the internal controls. ...
<p>The RT-PCR results from RNA isolated from PBMCs infected with HIV-1(MN) and HIV-2 (ROD) <i>(</i>A...
We conducted research on high-flux transcriptome regarding alternative polyadenylation and host gene...
<p>Twelve transcripts were selected for real-time RT-PCR to validate the expression patterns in diff...
<p>The heatmaps of 6,624 significant DEGs (left) and numbers of potential signature genes were group...
<p>(A) Real-Time RT-PCR analysis performed with RNA extracted from the PT4 cell culture containing G...
Murine L929 cells were infected for 19.5 h with reovirus isolates c87, c8 and Dearing or mock (M) in...
<p>(A) Scatter plot of global gene expression in the EtOH- and R5020- treated T47D cells. In total, ...
<p>All genes (A), up-regulated genes (B) and down-regulated genes at four time-points post-infection...
<p>Relative transcription of Th1, Th2, pro-inflammatory, anti-inflammatory, Th17 and regulatory cyto...
<p>Ten expression trend clusters of 699 DE genes derived from PAMs during PRRSV infection.</p
<p>Twelve expression levels of selected genes from the microarray assay were validated by real-time ...
<p>a. The ΔC<sub>T</sub> value is determined by subtracting the average Actin C<sub>T</sub> value fr...
1<p>fold change by real-time RT-PCR is relative gene expression level (chGH/PBS).</p>2<p>fold change...
<p>(A) (i) m-RNA expression (ii) quantification of Caspase 8, Bid, and Bax gene by RT-PCR in K562 ce...
<p>The relative expression levels were calculated using β-actin and GAPDH as the internal controls. ...
<p>The RT-PCR results from RNA isolated from PBMCs infected with HIV-1(MN) and HIV-2 (ROD) <i>(</i>A...