Expression of SERCA3 and PMCA4 at different time periods of infection.

<p>Macrophages (2×10⁶ cells) were infected with <i>L. donovani</i> (1∶10) and incubated at 37°C for 3, 6 and 12 hr. The cells were collected in Trizol for RNA extraction and semi-quantitative RT-PCR was performed. PMCA4, SERCA3 and GAPDH PCR products were resolved on an agarose gel (1.5%) and quantified densitometrically using lab software as described in Methods (A). In a separate set of experiment, cell lysates were prepared from infected macrophages, followed by Western blot for PMCA4, SERCA3 and GAPDH (B). Data shown above (A and B) is a representative one experiment, performed at least three times.