Relative transcription of <i>PmpD.</i>

<p>Total RNA was isolated from <i>C. trachomatis</i> serovar L2 infected McCoy cells at 0, 2, 4, 6, 8, 10, 12, 16, 20, 24, 30, 35, and 48 h p.i., random primed, and assayed by real time RT-PCR for 16S rRNA and <i>pmpD</i> copy number. Averaged <i>pmpD</i> copy numbers were indicated relative to the rRNA numbers at the same time points. Error bars were based on the standard error of the mean <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000568#pone.0000568-Hogan1" target="_blank">[26]</a>.