Screening of PTPs regulated in RAW 264.7 cells in response to LPS.

<p>Each cDNA was synthesized from total RNA that was extracted from RAW 264.7 cells at the indicated times after treatment with LPS (1 µg/ml). PTP transcripts were amplified using specific primers listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078776#pone-0078776-t001" target="_blank">Table 1</a>. GAPDH transcripts were amplified using specific primers (forward 5′-ACCACCATGGAGAAGGC-3′; reverse 5′-CTCAGTGTAGCCCAGGATGC- 3′) as a control. Similar results were obtained in three independent experiments. The PCR products were visualized by ethidium bromide staining and quantified by scanning the gel images followed by analysis with LabWorks software (UVP Inc.). The PCR data were normalized to the expression level of GAPDH and are presented as relative fold changes.