CPE activates ERK and AKT signaling pathways in primary cultured rat hippocampal neurons.

<p>Top panels: Representative Western blot analyses of hippocampal neuron lysates showing p-ERK 1/2 (<b>A</b>) and p-AKT (<b>B</b>) after 0, 15, 30 and 60 min treatment with 0.4 µM CPE. Total ERK (t-ERK 1/2) and total AKT (t-AKT) were also analyzed and served as internal controls. Bottom panels: Bar graphs showing the quantification of the p-ERK 1/2 (<b>A</b>) and p-AKT (<b>B</b>) signals normalized to t-ERK 1/2 and t-AKT, respectively. Note the increase in p-ERK and p-AKT after 15, 30 and 60 min treatment with CPE. Statistical analysis for <b>A</b> and <b>B</b> by one way ANOVA with Tukey post-hoc test, n = 4, *p<0.05 when compared to untreated control cells.