MEK and ERK signaling was up-regulated in the hippocampus of P8 BTBR mice, but not in the cortex.

<p>(<b>A</b>) Western blots using hippocampal lysates against p-MEK/MEK and p-ERK/ERK. After p-MEK was analyzed, the same membranes were stripped and used to analyze MEK. Finally, the membranes were stripped again and actin was probed. Same approach was used to analyze p-ERK and ERK. (<b>B</b>) Quantification of relative expression levels. The expression levels of p-MEK and MEK were normalized by actin, and the ratio of p-MEK to MEK was computed using the normalized relative expression levels of p-MEK and MEK. Same method was used to quantify p-ERK and ERK expression levels and the ratio of the two. (<b>C</b>) Western blots using cortical lysates against p-ERK and ERK. Same methods were used as in (<b>A</b>). (<b>D</b>) Quantification of relative expression levels of p-ERK and ERK in cortical lysates. *: <i>P</i> < 0.05, **: <i>P</i> < 0.01 by Student's <i>t</i> test. Vertical line in the image of (<b>A</b>) and (<b>C</b>) indicates the position of a lane that was loaded with markers of molecular weight and was cropped out.